Leucidal® Liquid Complete
Leucidal Liquid + AMTicide Coconut

ECOCERT/COSMOS Approved
Whole
Foods Compliant*
Click Here to View Antimicrobial Formulating Guidelines

Active Micro
Technologies prides themselves on developing and supplying effective,
natural products that deliver skin and hair conditioning
benefits, along with providing natural antimicrobial activity. As our original
antimicrobial product line and effective
antifungal
booster continue to lead the natural antimicrobial market, a convenient broad-spectrum antimicrobial
mixture with efficacy
against bacteria, yeast and mold has been developed to provide full protection
in one product. Leucidal® Liquid
Complete combines the
antibacterial power of Leucidal® Liquid and the antifungal power of AMTicide® Coconut to deliver an effective and
convenient one step solution! This highly marketable product can provide
moisturizing and conditioning benefits in hair
and skin care
applications. Leucidal® Liquid Complete is effective at preventing the
growth of bacteria and fungi, including yeast and mold,
making it the perfect addition to any formulation.

Leucidal® Liquid
Complete is
a mixture of antimicrobial peptide technology. One type of the antimicrobial
peptides is originally
derived from the lactic acid bacteria, Leuconostoc kimchii. L. kimchii is one of 15
species of microorganisms that
make up the
mixed culture used for producing the Korean dietary staple known as kimchi, a
type of fermented cabbage. L. kimchii is capable of
restricting the growth of other microorganisms by acidifying its environment,
but as is
common in
nature, it is not content to limit itself to a single mechanism of defense. In
addition to acidifying its
environment, it also produces a novel antimicrobial peptide. Using modern
fermentation and bioprocessing technology, Active
Micro
Technologies has commercialized this antimicrobial peptide to produce Leucidal® Liquid
Complete.
The other type
of antimicrobial peptide is created from fermenting Cocos nucifera (Coconut) with Lactobacillus. Coconut oil has
been traditionally used to treat skin disorders, yeast infections, ringworm,
and even athlete’s
foot. About 50%
of the total fatty acid content of coconut oil are medium chain triglycerides
(MCT’s) that exhibit natural
antifungal activity. MCT’s, such as lauric acid, work by disrupting the
cellular structures of fungus and
destroying them
before they can wreak havoc. During the fermentation process, lipopeptides are
catabolized by the MCT’s
present in coconut flesh. Active Micro Technologies has harnessed natural
phytocompounds and
lipopeptides
from coconut to produce a novel antifungal material.
Natural
antimicrobial products are similar to synthetic preservative systems in that
they are effective against bacteria,
however most natural antimicrobial products lack broad-spectrum effectiveness
against bacteria and
fungi,
specifically yeast and mold. Leucidal® Liquid Complete uses a combination of peptide technology to deliver moisturizing and conditioning benefits as well as
providing broad-spectrum activity to protect against
bacteria,
yeast, and mold in one product.

A skin
moisturization study was performed using an untreated control, generic cream
base, and an experimental
with the same cream base containing 2.0% Leucidal® Liquid Complete. The average increase
in moisturization is in Figure 1 below.

Comparative
moisturization results from this study are shown in Figure 2. As demonstrated
by the results of this study,
the addition of 2.0% Leucidal® Liquid Complete improved moisture levels by
42.01% after
24 hours and by
62.33% after four weeks when compared to the untreated control. When compared to the base
cream Leucidal®
Liquid Complete improved moisturization by 14.38% and after 24 hours
and by 24.13%
after four weeks. Based on these results, adding this innovative product
provides the formulator the
opportunity to capitalize on both the natural antimicrobial properties of Leucidal® Liquid
Complete, as well as
its ability to provide potent moisturizing benefits to the cosmetic
formulation. These
properties make it ideal for applications addressing numerous skin and scalp
conditions.

One of the
first steps in the development of Leucidal® Liquid Complete was to
determine the product’s potential ability to
inhibit the growth of a variety of bacteria and fungi. Using standard serial
dilution protocols in growth
media, the
Minimum Inhibitory Concentrations (MICs) for Leucidal® Liquid Complete were determined
for a variety of bacterial
and fungal organisms. The results of these tests are shown in Figure 3.

The positive
MIC screening results warranted further testing to confirm its ability to
provide product preservation. A
Double Challenge Test was completed using 2.0% Leucidal® Liquid
Complete in
a generic
cream base
formulation. Samples were inoculated with E. coli, P. aeruginosa, S. aureus, C. albicans, and A. brasiliensis. During the
first 28-day incubation period, samples were periodically collected and tested
for the
presence of
these microorganisms. Following this initial 28 days of incubation, the cream
samples were then re-inoculated
with the microbial cultures and sampled over an additional 28-day period.
Figure 4 shows the
positive
preservation results for Leucidal® Liquid Complete.


As with all
biological materials, attention must be paid to the conditions under which Leucidal® Liquid Complete is used. Based
on bench-scale evaluations, as well as actual product applications, Leucidal® Liquid
Complete has
been found to be effective over a wide range of
typical cosmetic and personal care product manufacturing conditions. Leucidal® Liquid
Complete has
been found to be heat stable up to70°C and active under both acidic (pH 3) and
basic (pH 8) conditions.
Store at or near room temperature; Prior to use, container must be warmed to 35 - 40°C and mixed until
clear. May sediment upon standing - Product
may change appearance if exposed to cold temperatures during shipment or
storage. If this happens, please gently warm to 45-50°C and mix until normal
appearance is restored.
INCI Nomenclature:
|
Leuconostoc/Radish Root
Ferment Filtrate
& Lactobacillus & Cocos
Nucifera (Coconut)
Fruit Extract
|
Suggested Use Levels:
|
2-4%
|
Appearance:
|
Hazy Liquid
|
Solubility:
|
Water Soluble
|
pH:
|
5.0-9.0
|
Storage: Protected from direct light and humidity at a temperature of
50°-77°F (10°-25°C)
Shelf life: 12 months, properly stored, in sealed container. This product should be added to a
formulation at the recommended usage rate