Leucidal® Liquid is a broad spectrum antimicrobial with moisturizing benefits.
Leucidal® Liquid
ECOCERT/COSMOS Approved
Whole Foods Compliant*
Australian Certified Organic
Leucidal® Liquid is on the Whole Foods Acceptable Premium Preservative List
Use with AMTicide Coconut – to protect against yeast and mold - Click here to View AMTiCide Coconut
Now Available - Leucidal Complete (Leucidal Liquid + AmtiCide Coconut)
For Further Information or Studies, Please request from us by e-mail
Leucidal® Liquid is based on an antimicrobial peptide originally derived from the lactic acid bacteria, Leuconostoc kimchii. L. kimchii is one of 15 species of microorganisms that make up the mixed culture used for producing the Korean dietary staple known as kimchi, a type of fermented cabbage.
Like many lactic acid bacteria, L. kimchii is capable of restricting the growth of other microorganisms by acidifying its environment, but as is common in nature, it is not content to limit itself to a single mechanism of defense. In addition to acidifying its environment, it also produces a novel antimicrobial peptide. Using modern fermentation and bioprocessing technology, AMT has commercialized this antimicrobial peptide to produce Leucidal® Liquid.
The first step in the development of this product was to determine the peptide’s potential ability to inhibit the growth of a variety of bacteria and fungi. Using standard serial dilution protocols in growth media, the Minimum Inhibitory Concentrations (MICs) for Leucidal® Liquid were determined for a variety of both bacterial and fungal organisms. The results of these tests are shown in Table 1.
The positive MIC screening results warranted further testing to confirm its ability to provide product preservation. A Double Challenge Test was completed using 2% Leucidal® Liquid in a generic cream base formulation. Samples were inoculated with E. coli, P. aeruginosa, S. aureus, K. pneumoniae, C. albicans, A. niger, and B. cepacia. During the first 28-day incubation period, samples were periodically collected and tested for the presence of these microorganisms. Following this initial 28 days of incubation, the cream samples were then re-inoculated with the microbial cultures and sampled over an additional 28-day period. Table 2 shows the positive preservation results for Leucidal® Liquid.
Because peptides have been found to provide skin moisturization properties, Leucidal® Liquid was used in a comparative study to evaluate its ability to provide cosmetic benefits, in addition to the demonstrated antimicrobial function. A skin moisturization study was performed using a generic cream base, compared with the same cream base containing 1% Leucidal® Liquid. As demonstrated by the results of this study, shown in Table 3, the addition of 1% Leucidal® Liquid to the base cream formulation provided a 10% increase in moisturization. Based on these results, adding this innovative product provides the formulator the opportunity to capitalize on both the natural antimicrobial properties of Leucidal® Liquid, as well as its ability to provide potent moisturizing benefits to the cosmetic formulation. These properties make it ideal for applications addressing numerous skin and scalp conditions.
As with all biological materials, some attention must paid to the conditions under which Leucidal® Liquid is used. Based on bench-scale evaluations, as well as actual product applications, Leucidal® Liquid has been found to be effective over a wide range of typical cosmetic and personal care product manufacturing conditions. The product has been found to be heat stable up to 70°C and active under both acidic (pH 3) and basic conditions (pH 8).
Quick Tips when using Leucidal® Liquid:
1. We recommend starting with 4.0% Leucidal® Liquid.
2. Monitor the interaction of Leucidal® Liquid with other ingredients for compatibility.
3. Incorporate Leucidal® Liquid into formulations at a pH between 3 and 8, during the cooling phase of the process at temperatures lower than 70°C
Heat Stability
Results from testing has shown that Leucidal® Liquid can tolerate temperatures of up to 70°C for a period of 8 hours without having any impact on MIC performance.
Compatibility
The cationic nature of Leucidal® Liquid makes the main formulating concern potential incompatibility with highly anionic ingredients, such as thickeners. Please request from us for more information regarding specific anionic thickeners. The use of solubilizing agents may help improve clarity in cases where haziness is observed.
Recommended Techniques
Incorporating Leucidal® Liquid towards the end of the formulating process: A successful recommendation is adding Leucidal® Liquid near the end of the formulating process and after the formulation has cooled to below 70°C. Please request from us for recommended techniques regarding specific anionic thickeners.
Additional Information
Leucidal® Liquid has been in use for almost a decade now and has been proven to be a very compatible ingredient for formulating.
We always recommend that every formula undergo stability and microbial testing to ensure adequate preservation.
INCI Nomenclature:
Leuconostoc/Radish Root Ferment Filtrate
INCI Status: Approved
REACH Status: Fully Compliant
CAS Number: 84775-94-0
EINECS Number: 283-918-6
Origin: Biotechnology/Botanical:
Leuconostoc kimchii & Raphanus Sativus
Processing:
GMO Free
No Ethoxylation
No Irradiation
No Sulphonation
No Ethylene Oxide treatment
No Hydrogenation
Additives: None
-Preservatives: None
-Antioxidants: None
Other additives: None
Solvents used: Water
Appearance: Clear to Slightly Hazy Liquid
Soluble/Miscible: Aqueous Ferment
Extract
Suggested Use Levels: 2.0 - 4.0%
Suggested Applications:
Moisturization,Skin/Scalp Conditioning,
Antimicrobial
We always recommend that every formula undergo stability and microbial testing to ensure adequate preservation.
INCI Nomenclature: Leuconostoc/Radish Root Ferment Filtrate
Appearance: Clear to Slightly Hazy Liquid
Solubility: Water Soluble
Suggested Use Levels: 2.0-4.0%
Suggested Applications:Moisturization,Skin/Scalp Conditioning, Antimicrobial.
Storage: Protected from direct light and humidity at a temperature of 50°-77°F (10°-25°C)
Shelf life: 12 months, properly stored, in sealed container.
This product should be added to a formulation at the recommended usage rate.
All products sold are cosmetic grade; External Use Only; Cosmetic & Personal Care Formulations Only.
Our products are for cosmetic and personal care formulations only; External Use Only. We recommend working with a chemist or a formulator if you’re not advanced in formulating.
Statements contained herein have not been evaluated by the Food and Drug Administration. These products are not intended to diagnose, treat and cure, or prevent disease.
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